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Part:BBa_K1758204:Design

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-29)


His-tagged blc repressor fused to superfolder GFP under the control of the T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 800
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 508
    Illegal NgoMIV site found at 749
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 893


Design Notes

This is a composite part which enables a high protein expression and an easy purification of blcR fused to superfolding GFP (sfGFP). The green fluorescence of the culture is a sign for protein expression.


Source

We clone blcR in T7-RBS-sfGFP and added a His-Tag via PCR and Gibson Assembly.

References